Recombinant DIABLO antibody (C-Term)

Catalog No. ABIN4953421

The Rabbit Monoclonal anti-DIABLO antibody has been validated for WB and IHC. It is suitable to detect DIABLO in samples from Human.

Quick Overview for Recombinant DIABLO antibody (C-Term) (ABIN4953421)

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DIABLO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RM271

Product Details anti-DIABLO Antibody

Binding Specificity: C-Term

Characteristics: This recombinant Smac antibody reacts to human Smac (Diablo homolog, mitochondrial).

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A peptide corresponding to the C-terminus of human Smac/Diablo was used as the immunogen for this recombinant Smac antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Smac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. IHC (FFPE): 1:500-1:1000 (1),Western Blot: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant Smac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: Smac / Diablo

Background: This recombinant Smac antibody reacts to human Smac (Diablo homolog, mitochondrial).

Gene ID: 56616

UniProt: Q9NR28

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity